Pmutin-gfp+
WebJan 29, 2016 · Two separate plasmids namely pMUTIN-GFP+ and pDG1662 were used for the construction of plasmid pDG1662-A. On isolation of genomic DNA from B. subtilis … WebDec 1, 1998 · The integration of a pMUTIN vector into the target gene has three consequences: (1) the target gene is inactivated; (2) lacZ becomes transcriptionally fused …
Pmutin-gfp+
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http://muchong.com/t-5006068-2 WebJan 1, 2016 · Transformants were PCR confirmed using pMUTIN-GFP gene specific primers ( Table S1) that were designed from gfp gene sequences for amplification of 359 bp. PCR was performed in GoTaq PCR kit of Promega (GoTaq Buffer-1X, MgCl 2 – 1.5 mmol l −1, dNTPs – 200 μmol l −1, Forward/Reverse primers – 10 pmol each, DMSO – 6%, Taq …
WebpMUTIN-GFP+ Erythromycin selectable in EC or BS; Ampicillin selectable only in EC; GFP+ full coding sequence, including RBS and Pspac; IPTG-inducible; full plasmid insertion WebApr 8, 2005 · Plasmid pMUTIN-GFP+ is a cloning vector (based on a ColE1-like replicon) designed for construction and expression of fusion genes encoding hybrid proteins composed of N-terminal part of the desired protein (or. PAP I leader sequence directs the protein to cellular membrane.
WebMay 28, 1998 · Plasmid pMUTIN-GFP+ is a cloning vector (based on a ColE1-like replicon) designed for construction and expression of fusion genes encoding hybrid proteins composed of N-terminal part of the desired protein (or its part) and green fluorescent protein (GFP) [18]. Show abstract. WebFeb 15, 2007 · A variant of the green fluorescent protein, GFP+ (here referred to as GFP), exhibiting a faster folding and an increased fluorescence compared to the wildtype was used as reporter since it can be...
Web你好 师姐 你有pmutin质粒吗？ 师妹最近也在做芽孢的敲除实验 但是一直做了4个月了 都毫无收获 现在已经研三了 可以把你的质粒卖给我吗 谢谢 赞 一下
WebThe invention discloses bacillus gene traceless knockout/enter plasmid, method and kit, contain in the genome of the bacillus gene traceless knockout/enter plasmid：Only a kind of resistant gene of... purple gray and black area rugWebThe homologous recombination fragment was inserted into the integration vector pMUTIN-GFP+, which could integrate into chromosome by a single recombination event, to construct knockout plasmid. securian state of tnWeb(pMutin-GFP+) Ap: GM2163(pMutin-GFP+) Shipping Conditions: LB agar with ampicillin (100 µg/ml) Growth Conditions: LB agar with ampicillin (100 µg/ml) Temperature: 37: References: Kaltwasser M, Wiegert T, Schumann W. Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis. Appl ... purple gray black and white living room ideasWebDownload scientific diagram HD-73 strain of Bacillus thuringiensis transformed with the pMUTIN-gfp vector, containing the gpf gene under the control of the pspac promoter (Bt … purple graveyard aestheticWeb(pMutin-GFP+) Ap: GM2163(pMutin-GFP+) Shipping Conditions: LB agar with ampicillin (100 µg/ml) Growth Conditions: LB agar with ampicillin (100 µg/ml) Temperature: 37: … purple graphic shirtsWebThe plasmid, pKW244, carrying the mcpB-gfpmut3 fusion was built in three steps. First, the gfpmut3 gene was amplified by PCR using pGFPmut3 ( 34 ) as the template and inserted into the ClaI and SpeI restriction sites of plasmid pMUTIN-GFP+ ( 35 ), replacing gfp with gfpmut3 and yielding the plasmid pKW235. securian s\\u0026p 500 index tickerWebFeb 3, 2011 · For FACS experiments performed 7–14 days after muscle dissociation, cultured nestin-GFP+ cells were washed with phosphate-buffered saline (PBS) and treated with 0.25% trypsin/0.05% EDTA to isolate them in a suspension. When all cells were detached, the enzymatic reaction was stopped with the resuspension medium described … purple graphic t shirts