Phic31 attb

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August undefined, 2024

WebBackbone manufacturer Stratagene Vector type Mammalian Expression, Bacterial Expression, Mouse Targeting ; att/PhiC31 Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 30°C Growth Strain (s) DH10B Copy number High Copy Gene/Insert Gene/Insert name Neo* …

Why PhiC31? - FlyC31

WebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between … destiny 2 shipyard awo-43

PhiC31 Integrase Expression Plasmid System Biosciences

WebThe forward and reverse primers will be designed to incorporate attB1 and attB2 sites respectively, to allow clonase-mediated integration of the PCR product into a Gateway entry vector. The primers will be designed to both have a … WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebphiC31 Integrase Vector System www.systembio.com/phic31 One-step gene addition technology Nonviral transgene delivery The phiC31 integrase is a sequence-specific … chuffin puffin

Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and …

Nucleofection of phiC31 Integrase Protein Mediates Sequence ... - PubMed

WebSekelsky lab Drosophila phiC31 cloning plasmids w+attB Sequences w+attB Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Full (1) Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. WebDuring pUAST/PhiC31 integrase-mediated integration, the unique attP site in the ZH-attP-51D fly line genome recombines with the pUAST attB site in the pUAST-PrP vector forming attR and attL... chuffle song 2011WebSep 8, 2013 · Phage PhiC31 integrase integrates attB-containing plasmid into pseudo attP site in eukaryotic genomes in a unidirectional site-specific manner and maintains robust transgene expression. Few studies, however, explore its potential in livestock. This study aims to discover the molecular basis of PhiC31 integrase-mediated site-specific … destiny 2 shipstealer

"WebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … " - Phic31 attb

Phic31 attb

Christopher Chavez, PhD (INTP-T) - LinkedIn

WebJun 12, 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP. WebMay 3, 2007 · Under in vitro conditions ϕC31 integrase converts ∼80% of attB and attP to products in the absence of accessory proteins and there are no restrictions on the topology of the substrates ( 16, 27, 28 ). Moreover, in these in vitro reactions, ϕC31 integrase is catalytically inert on all other combinations of substrates including attL and attR ( 28 ).

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WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB and attP 2, 3. PhiC31 integrase does... WebOct 28, 2024 · 7, when a cell containing the variant phiC31 integrase that recognizes the pseudo-attP site, a recombination between the pseudo-phiC31 attP site and the phiC31 attB site occurs and the orientation of the CMV promoter in the selection construct is reversed. The reversed CMV promoter then drives the expression of the GFP reporter gene.

WebFeb 15, 2024 · The attB site (5'-TGCGGGTGCCAGGGCGTGCCCttGGGCTCCCCGGGCGCGTACTCC-3') is one of the two … http://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf

WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ...

WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … chuffley light railwayWebThe phiC31 integrase catalyzes unidirectional recombination between two attachment sites, attP and attB, leading to the formation of attL and attR sites . A circular construct harboring an attB site can be efficiently and specifically integrated into … chuffle dance modern talking remixWebJul 21, 2006 · The stability of the attB × attP locus, well-documented with the phiC31 integrase system in bacteria and yeast 16,17, was evidenced here by the lack of rearrangement or reversion to the wild-type ... chuffle foodWebFor installation in small housings, control cabinets and operator panels. Simple and fast wiring by means of screw terminals. Space-saving design. For integration of mechanical … destiny 2 shiro-4WebJan 1, 2013 · Background: PhiC31 integrase is a DNA site-specific recombinase integrates DNA into the chromosomes between the two sites of attB and attP. Several pseudo attPs have been identified in mammalian ... chuff lifeWebCall us today at (312) 466-9466 to lean about this important criminal defense option. You may be eligible for a deferred prosecution program if you are arrested in the Chicagoland … destiny 2 shitpostWebMar 21, 2012 · Depending on the relative orientation of the attP and attB sites, the reaction can result in excision, inversion or integration of sequences between the recognition sites, and is not reversible unless an additional protein, an excisionase, is present. chuffling