Pegfp c1 empty vector
WebJan 9, 2012 · Tubular cell HIV-infection has been reported to manifest in the form of cellular hypertrophy and apoptosis. In the present study, we evaluated the role of mammalian target of rapamycin (mTOR) pathway in the HIV induction of tubular cell protein synthesis. Mouse proximal tubular epithelial cells (MPTECs) were transduced with either gag/pol-deleted … WebRK2载体价格3800元，RK2载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄、卡那、四环素，质粒大小为56 kb。RK2载体是质粒。
Pegfp c1 empty vector
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WebAug 14, 2015 · zt1208 pEBFP-C1 . zt1209 pECP4 . zt1210 pEF1a-hyPBase(2000) zt1211 pEF-Flag . zt1212 pEGFP-C2 . zt1213 pEGFP-K48 . zt1214 pEGFP-K63 . zt1215 pEGFP-LC3 . zt1216 pEGFP-ub . zt1217 pEYFP-N1 . zt1218 pF25A ICE T7 Flexi Vector . zt1219 pGBK-lam WebThe pEGFP-C1 (empty vector) transfected cell line was used as a control. Transfection efficiency of each group was >90% and transfected cells expressed functional SR-AI protein.
WebThe influence of BMP-2 on cell migration and cofilin activity was detected by cell scratch assay and Western blotting. Results: The recombinant pEGFP-C1-BMP-2 was effectively expressed in COS-7 cells. An increased phosphorylation of both LIMK1 and cofilin and an enhancement of cell migration were observed in cells with overexpression of BMP-2. WebThe Invitrogen Vivid Colors Fluorescent Protein Vectors are our next-generation cloning and expression tools featuring Aequoreavictoria -derived fluorescent proteins (FP) for simple, non-invasive detection of recombinant proteins. With Invitrogen Gateway and TOPO cloning vector options, cloning, expression, and viewing has never been so easy!
WebNot1-digested PEGFP-N1 mammalian expression vector making use of T4 DNA ligase. This vector was then sub-jected to colony-PCR as well as DNA sequencing for the accurate assessment of the sub-cloning processes. Ligation After confirmation, the MOG gene was prepared to be li-gated to expression vector. Clean up gene kit was used to WebDec 23, 2024 · HEK293 cell lines co-transfected with pECFP-N1 and pEYFP-C1 were set as a negative control (NC). ... we used the T2A peptide to construct an eukaryotic expression vector pEYFP-EspF-T2A-ANXA6 ... or/and pEYFP-ANXA6-HA (1 μg); pEGFP-N1 was set as an empty plasmid control. After 48 h from transfection, cell monolayers were washed with …
WebGreen fluorescence in SUVEC cells transfected with pEGFP-C1-MELK and pEGFP-C1 (empty vector) after 24 h was detected by fluorescence microscopy, whereas no fluorescent signals were detected...
WebApr 11, 2024 · The control vector pEGFP-C1 and six protein-expressing plasmids were transfected into RD cells, respectively, followed by incubation with tested compounds for 24 h, the fluorescence intensities of the cells were observed and photographed by fluorescence microscope (Magnification 20×) (Fig. 5). Compared with the cell control group, the ... sugar rush rancis fluggerbutterWebNov 4, 2024 · As expected, we found that SPIN1 selectively interacted with GFP-SPINDOC, but not with the pEGFP-C1 empty vector control. We note that GFP-SPINDOC can enrich for SPINDOC itself, possible due to... paint with stencilWebPEGFP-C1 encodes a red-shifted variant of wild-type GFP, which has been optimized for brighter fluorescence and higher expression in mammalian cells. (maximum excitation = 488 nm; maximum emission = 507 nm) pEGFP-C1 encodes GFPmut1 variant, which contains Phe-64 pairs of Leu and Ser-65 to Thr double amino acids instead of MCS in pEGFPC1. sugar rush raleighWebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of … sugar rush racers swizzleWebVector Database. Digital collection of empty plasmid backbones from publications and commercially available sources. Help Center . Ordering & MTAs. ... pEGFP-C1-3x-NLS-mScarlet-AMPKα2. Plasmid #192452. Purpose. mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus. Depositor. paint with stitchesWebTransfection and selection data related to pEGFP-C1 in cell-culture. sugar rush racers taffytaWebAcGFP1 is a monomer, which makes it a superior alternative for fusion applications. EGFP has been reported to form dimers (Jain et al. 2001; Chen et al. 2002). Another alternative to EGFP is ZsGreen1, a very bright, tetrameric green … paint with stencils on wood