Dsrna j2
WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … WebDescription: Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. …
Dsrna j2
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Web5 ott 2014 · J2 dsRNA pull-down. J2 antibody (Scicon, 10010200, diluted to 0.1 μg per 1 μg of chromatin) was incubated with total cell extracts for 1.5 h on a rotating wheel at 4 °C. WebThe original J2 antibody was raised by immunising mice with L-dsRNA. Conjugate. Unconjugated ... and virus management. In this study, we have attempted to engineer resistance againstM. incognitaand ToLCV by stacking dsRNA constructs of theIntegrasegene ofM. incognitaandAC4genes of ToLCV, using co-transformation …
WebThe J2 anti-dsRNA IgG2a monoclonal antibody has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, ... WebJournal: BMC Cancer Article Title: Replicase-based plasmid DNA shows anti-tumor activity doi: 10.1186/1471-2407-11-110 Article Snippet: Monoclonal anti-dsRNA antibody J2 (English & Scientific Consulting Bt. Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Amplification, Enzyme-linked Immunosorbent Assay, …
Web5 dic 2015 · Immunofluorescence. differenttimes posttransfection, cells were washed twice phosphate-bufferedsaline (PBS) lacking Ca 20min methanol-acetone(1:1) cellswere air dried fewminutes roomtemperature prior primarymouse antibody, J2 monoclonal antibody (MAb J2; Engscicons), which directedagainst double-stranded RNA (dsRNA) longer than 40 … WebSpecificity / Sensitivity. dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6).
WebAlternative Name(s) of Target: double-stranded RNA Immunogen: The original J2 antibody was raised by immunising mice with L-dsRNA. Specificity: This antibody binds to dsRNA > 40 bp in length. Application Notes: This recombinant mouse monoclonal antibody, rJ2, is useful for detecting double stranded RNA of greater than 40 bp in length which is …
WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … how many times has the chief won super bowlWeb1 lug 2024 · Anti-dsRNA-specific J2 antibody (Scion) is widely used to recognize viral or cellular dsRNA of more than 40-bp length with no sequence specificity . First, we validated that the J2 antibody specifically recognizes authentic cellular dsRNAs by treating MCF7 cells, or isolated total RNAs, with RNases or DNase I, and performing immunostaining or … how many times has the bible rewrittenWebThe monoclonal anti-dsRNA antibody J2 binds double-stranded RNAs (dsRNA) in an apparently sequence-nonspecific way. The mAb only recognizes antigens with double-stranded regions of at least 40 bp and its affinity to poly(A) poly(U) and to dsRNAs with mixed base pair composition is about tenfold high … how many times has the bengals won super bowlWebHi, I am recently using the J2 antibody (SCICONS) to stain dsRNA in human dermal fibroblasts treated with or without poly(i:c). It was expected that cells treated with … how many times has the doctor regeneratedWebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic … how many times has the bible been alteredWeb4 apr 2024 · IHC staining for dsRNA using the dsRNA antibodies K1 and J2 provided positive results in the CNS of all tested animals (Appendix Table 1). Immunolabeling with anti-dsRNA antibody 9D5 remained negative for all 3 RusV-positive animals, whereas the RusV-negative lions 7 and 9 tested positive (Appendix Table 1). how many times has the earth flippedWebAnti Dsrna Specific J2 Igg2 A Monoclonal Antibody, supplied by SCICONS Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more how many times has the bible been revised